The current researches of Gobang man-machine game are mostly based on the computer, mobile phone, which are lacking real environments. In order to solve the problem, a game algorithm based on Laboratory Virtual Instrument Engineering Workbench (LabVIEW) was proposed, and was applied to Gobang man-machine game in real environment. Firstly, the state information of the chess board and the man-machine chess pieces location on both sides in the current state were obtained by the image acquisition system. Then the game situation was analyzed. In order to improve the efficiency of chess, the chess type was classified, and the original game algorithm was improved by using two weights of attack and defense to simplify the decision-making process. The experimental results of real game tests prove that the proposed algorithm based on LabVIEW can realize the Gobang man-machine chess fast and accurately.